drug administration 126 cape  (MedChemExpress)

Journal: bioRxiv

Article Title: The adenovirus E4orf1 protein initiates a feedback loop involving insulin and growth factor receptors, AKT, and NF-κB, leading to abnormal DNA content in infected cells

doi: 10.1101/2025.05.12.653445

Figure Lengend Snippet: NF-κB mediates the phosphorylation of the IGF1R and AKT in Ad-infected cells. (A) HeLa and (B) A549 cells were infected with the indicated viruses, and four hpi were exposed to AG1024, AG1478, an NF-κB nuclear translocation inhibitor, caffeic acid phenethyl ester (CAPE) , or the NF-κB transcriptional inhibitor, SC75741 (SC) , at the indicated concentrations for 48 hours. The lysed cells were analyzed by immunoblot probing for pAkt S473, pAkt T308, NF-κB p50 S337, pS6 S235/S236, pCdk2 T160, pERK1/2 T202/Y204, cyclin B, cyclin E, DBP and β-tubulin. (C) Empty pBabe vector- and Ad5 E4orf1-expressing MCF10A cells were exposed either to SC, CAPE, Torin-1, or LY294002, and 48 hours after analyzed by immunoblot probing for either pAkt S473, pAkt T308, or β-tubulin. (D) HeLa and (E) A549 cells were infected with the indicated viruses, and four hpi were exposed to AG1024, AG1478, SC, or CAPE at the indicated concentrations for 48 hours. The lysed cells were analyzed by immunoblot probing for IGF1R, pIGF1R Tyr1135/1136. The normalized pIGF1R intensity values were plotted in GraphPad prism and are shown.

Article Snippet: The pharmacological inhibitors used in this study were Torin1 (Cayman Chemical Company Cat #: 10997), LY294002 (Selleckchem Cat #: S1105), Akt inhibitor IV (Millipore Cat #: 124011), SC75741 (Selleckchem Cat #: S7273), AG-1478 (Selleckchem Cat #: S2728), AG-1024 (Selleckchem Cat #: S1234), Caffeic Acid Phenethyl Ester (CAPE) (Selleckchem Cat #: 7414), and rapamycin (Selleckchem Cat #: S1039).

Techniques: Phospho-proteomics, Infection, Translocation Assay, Western Blot, Plasmid Preparation, Expressing